Figure 2. TyrR^Gal4 mutant showed enhanced courtship behavior.

(A) Chaining indices for groups of males of the indicated genotypes. The “wild type” +/+ flies used here and in other panels are w⁺ flies that have been outcrossed to w¹¹¹⁸ for five generations. p<0.01; p<0.001 compared to control. n=7–10.

(B) A snapshot from Movie S1 showing male-male chaining exhibited by TyrR^Gal4 (bottom) but not the heterozygous control (top).

(C) Courtship indices using one target (w¹¹¹⁸) male and one tester male of the indicated genotypes. p<0.001 compared to the TyrR^Gal4/+ control. n=24–29 trials/genotype.

(D) Testing sexual preference. Single TyrR^Gal4/+ and TyrR^Gal4 males were tested for courtship behavior towards a single decapitated female and a single decapitated male. p<0.001 compared to the other gender. n=24.

(E) Single TyrR^Gal4/+ and TyrR^Gal4 males were tested for courtship behavior towards a single w¹¹¹⁸ female and a single fru^M null (fru^LexA/fru^4–40) male. p<0.001 compared to the other gender. n=24.

(F) Courtship indices using different age groups of w¹¹¹⁸ females and heterozygous or TyrR^Gal4 homozygous males. The ages of the target females are indicated. n=13–15 trials/genotype.

The error bars depict the means ±SEMs. All analyses of two samples employed Mann-Whitney tests. To analyze multiple samples we used the Kruskal-Wallis and Dunn’s post hoc test. **p<0.01. ***p<0.001.

See also Figure S1.