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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Vascul Pharmacol. 2016 Jul 8;85:29–38. doi: 10.1016/j.vph.2016.07.004

Figure 4. SFN reduces endothelial cell expression of adhesion molecules.

Figure 4

Surface expression of A + B) E-selectin and C + D) VCAM-1 were investigated using immune-fluorescent staining of non-permeabilized HBMEC-3 cells following SFN pre-treatment (1, 10 or 100 µM), and 4 h of saline or LPS (500 ng/ml) challenge. Staining was quantified as mean fluorescent intensity and normalized to account for cell number using the mean fluorescent intensity of DAPI staining. Scale bars = 10 µm. Data are mean ± SEM. n = 3 independent experiments for each treatment group. *p < 0.05, **p < 0.001 & ***p < 0.0001 vs. saline. #p < 0.05 vs. LPS.