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. 2016 Jun 15;5(8):615–625. doi: 10.1016/j.molmet.2016.06.005

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effect of miR-125b-5p LNA inhibitor injection in scWAT. 10 weeks-old C57BL/6 male mice received miR-125b-5p or control LNA inhibitor in the subcutaneous WAT and received the β3-adrenergic receptor agonist (CL316,243) for 7 days. miR-125b-5p expression levels were determined in scWAT from mice by RT-qPCR (A). Representative brite adipocytes, mitochondrial (B, C, E), and white adipocyte (D) markers mRNA levels were determined in scWAT by RT-qPCR. Representative histological sections of scWAT (7 μm, paraffin-embedded) HES staining (upper panel), UCP1 (middle panel) and PLIN1 (lower panel) immunostaining are shown (F). *: p < 0.05. Scale bar: 100 μm.