Figure 3.

Deformability of villin^−/−islets. (A) Deformability of mouse islets treated with or without latrunculin A (200 nM). (B) Deformation of villin^+/+ and villin^−/− islets in the reservoir. (C) Images of single villin^+/+ and villin^−/− islet cells in the reservoir or in the constricted channel of the microfluidic chamber. (D) Scatterplot of villin^+/+ and villin^−/− islet cell deformation versus cell size. Contour lines representing 95% and 50% of the maximum cell density are shown as solid and dashed lines, respectively. The size distribution and deformability distribution are shown in the top and right panels, respectively, in A, B, and D.