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. 2016 Sep 14;7:165. doi: 10.3389/fgene.2016.00165

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Copyright © 2016 Kramer, Jung, Hamann and Osiewacz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Arginine-specific peptidase activity [relative fluorescence units (RFU)/(min^*mg)] in total protein extracts of mycelium of 11 d old wild type, PaCypD_OEx and the different deletion strains. As substrate, the fluorochrome-coupled peptide Z-GGR-Amc was used. Fifty micrograms total protein extracts of four different isolates of each strain were measured. (B) Relative metacaspase activity was determined as difference of the arginine-specific peptidase activity from Panel A between wild type and ΔPaMca1/ΔPaMca2 or PaCypD_OEx and PaCypD_OEx/ΔPaMca1/ΔPaMca2. The mean metacaspase activity of the wild type was set to 100%. (C) Growth rate and (D) lifespan of 20 different isolates of the wild type, PaCypD_OEx, ΔPaMca1/ΔPaMca2, and the triple mutant PaCypD_OEx/ΔPaMca1/ΔPaMca2 on M2 medium. Data represent average ± SEM (2-tailed student's t-test), ^**P < 0.01, ^***P < 0.001.