Skip to main content
. 2016 Jul 7;5(8):602–614. doi: 10.1016/j.molmet.2016.06.016

Figure 1.

Figure 1

Drosophila MPC1 mutants are hyperglycemic and sensitive to dietary sugar. A: dMPC1 mutants die more rapidly as dietary sugar is increased. The percentage of surviving adult control (left panel) or dMPC1 mutants (right panel) fed a low (2% sucrose, 10% yeast; purple lines) or high (18% sucrose, 10% yeast; light blue lines) sugar diet was assayed every 2–5 days. N > 115 male flies per genotype under each feeding condition. Mean ± SEM is shown. B–E: Control (blue) or dMPC1 mutants (red) were aged 8–12 days on the low or high sugar diet and whole animal metabolite levels were measured to determine the abundance of B: trehalose C: glucose, D: glycogen, or E: triglycerides (TAG), all normalized to protein. N = 5–6 samples per genotype. Mean ± SEM is shown. *p < 0.05, **p < 0.01, and ***p < 0.001. F: Hemolymph glucose concentrations were determined in control and dMPC1 mutants aged 10 days on the high sugar media. N = 4 per genotype. **p < 0.01 G: Control and dMPC1 mutants were aged 5–9 days on standard laboratory media and fasted overnight for 16 h. After fasting, whole-animal glucose levels were determined and normalized to protein. N = 5 per genotype. **p < 0.01.