Fig. 6.

Fibrillization kinetics of Aβ42 incubated with and without Hoechst 34580 or Hoechst 33342. a Various concentrations (0.01–100 μM) of Hoechst 34580 was incubated with 50 μM Aβ42 at 37 °C for 70 h; b Various concentrations (0.01–100 μM) of Hoechst 33342 was incubated with 50 μM Aβ42 at 37 °C for 70 h; c Variation in ThT fluorescence intensity as a function of Hoechst 34580 (solid line) and Hoechst 33342 (dashed line). The data were subtracted to background from compound alone. Data were analyzed using GraphPad Prism to obtain IC₅₀ values using log (inhibitor) versus normalized response-variable slope. Dose–response curves showed fractional binding of 5 μM ThT to 50 μM Aβ42 fibrils in the presence of Hoechst 34580 or Hoechst 33342, respectively