Table 1.
Primers details and amplicon sizes used in multiplex HRMA
| Set no. | Primer name | Gene | Sequence (5′→3′) | Amplicon size (bp) | Amount of primers per reaction (pmol) |
|---|---|---|---|---|---|
| 1 | Exon6_F | TPMT | TGTTGAAGTACCAGCATGCAC | 173 | 2.75 |
| Exon6_R | CTTACCATTTGCGATCACCTG | 2.75 | |||
| Exon9_F | GAATCCCTGATGTCATTCTTCA | 213 | 11.0 | ||
| Exon9_R | CCTCAAAAACATGTCAGTGTGA | 11.0 | |||
| 2 | Exon4_F | CCCTCTATTTAGTCATTTGAAAACA | 241 | 11.0 | |
| Exon4_R | AAAACTTTTGTGGGGATATGGA | 11.0 | |||
| Exon2_F | ITPA | TAGGAGATGGGCAGCAGAGT | 163 | 6.75 | |
| Exon2_R | TCAATTTTCTGTGCCACCAA | 6.75 |
HRMA high resolution melting analysis, F forward, R reverse