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. 2016 Aug 26;6:33–38. doi: 10.1016/j.lrr.2016.08.001

Fig. 2.

Fig. 2

The protein expressions of P-gp and CD147 in drug-resistant K562/ADR and drug-free K562/ADR leukemic cell lines. Proteins were extracted drug-resistant K562/ADR and drug-free K562/ADR cell lines and separated by 12% SDS-PAGE and transferred onto PVDF membrane. The membranes were probed with anti-CD147, anti-P-gp and detected by enhanced chemiluminescence. (A) Results are representative of three independent experiments by Western blotting and (B) Quantification of P-gp and CD147 expressions in drug-resistant K562/ADR and drug-free K562/ADR cells; the protein levels were normalized to the loading control GAPDH. The ratio of P-gp and CD147 were calculated for each group. Data are presented as mean±SEM of three independent experiments; ⁎p<0.05 when compared to drug-free K562/ADR cell.