TABLE 1 .
Dimer plasmid DNA transformation efficiencies
| Temp (ºC), condition, and DNA type | Transformation efficiencya |
|
|---|---|---|
| Red | RecET | |
| 32, uninduced | ||
| Supercoiled | 3.5 | 4.8 |
| Linear | 0.021 | 0.013 |
| 42, induced | ||
| Supercoiled | 1.6 | 2.9 |
| Linear | 0.70 | 1.04 |
a
Transformation efficiency is expressed as the percentage of viable cells that were Ampr transformants. The values obtained with the supercoiled dimer control plasmid are presented for comparison with those obtained with the linear dimer plasmid. The number of replicates in each experiment ranged between four and seven, with an average standard deviation of 49%.