TABLE 2 .
Recombinant plasmids formed from linear dimer transformation
| Active recombination system | No. of plasmids/total (%) |
||||
|---|---|---|---|---|---|
| Monomersa | Symmetric resection (tet-14)a | Resection from left (tet-10 tet-14) | Resection from right (Tet+) | Other (tet-10) | |
| Red | 55/60 (91.7) | 6/55 (10.9) | 24/55 (43.6) | 25/55 (45.5) | 0/55 |
| RecET | 61/84 (72.6) | 19/61 (31.1) | 26/61 (42.6) | 15/61 (24.6) | 1/61 (1.6) |
a
The number of higher-order forms is significantly higher for RecET (P = 0.0051 [Fisher exact test]). The tet-14 class is significantly higher for RecET (P = 0.012 [Fisher exact test]).