TABLE 3 .
E. coli K-12 strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | Invitrogen |
| DH10B | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG λ− tonA | Invitrogen |
| HME6 | W3110 Δ(argF-lac)U169 galKtyr145UAG [λcI857 Δ(cro-bioA)] | 3 |
| HME68 | W3110 Δ(argF-lac)U169 galKtyr145UAG mutS<>cat [λcI857 Δ(cro-bioA)] | 41 |
| HME69 | W3110 Δ(argF-lac)U169 galKtyr145UAG mutS<>cat [λcI857 Δ(cro-bioA)] polA resA1::Tn10 | 34 |
| HME71 | HME6 Δ(srlA-recA)::Tn10 | 41 |
| LT1712 | SIMD89 polA resA1::Tn10 | This study |
| LT1714 | W3110 mutS<>cat | This study |
| LT1715 | W3110 mutS<>cat polA resA1::Tn10 | This study |
| LT1533 | DH10B mutS<>cat | This study |
| SIMD89 | HME6 [λ(int-cIII) recT] mutS<>cat | 17 |
| SIMD95 | HME6 [λ(int-cIII) gam recEa recT] Δ(srlA-recA)::Tn10 | This study |
| SIMD99 | HME6 [λ(int-cIII) gam recEa recT] recA<>speC | This study |
| SIMD101 | HME6 recA<>speC | This study |
a
The recE gene is full length, encoding a protein of 866 amino acids.