FIG 2 .

Label-free quantitation of proteins identified by shotgun proteomic analysis in conditioned medium from C. albicans cultures grown under biofilm and planktonic conditions. (A, left) Volcano plot depicting the log₂-fold change in protein abundance (reported as biofilm/planktonic), demonstrating a significant increase in Sap5 and Sap6 levels under biofilm conditions with a log₂-fold change of 3.1 for Sap5 (P =3.7 × 10⁻⁴) and a log₂-fold change of 5.3 for Sap6 (P = 9.1 × 10⁻⁵). (A, right) Corresponding histogram showing the relative distribution of proteins identified. (B) Comparison of relative Sap5 and Sap6 peptide levels under biofilm and planktonic conditions with mean log₂-fold changes indicated. Each data point represents quantification of a unique tryptic peptide for Sap5 or Sap6. Mean values of triplicate samples are reported. Supporting proteomic data are provided in Data Set S1 in the supplemental material.