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. 2016 Aug 31;2016:4378461. doi: 10.1155/2016/4378461

Figure 3.

Figure 3

Apigenin increases the expression of Nrf2 target genes involved in antioxidant system in ARPE-19 cells. (a) Real-time PCR analyses of the mRNA expression of HO-1, NQO1, and GCLM in t-BHP-treated ARPE-19 cells. Significance: ∗ P < 0.05 versus control, ∗∗ P < 0.01 versus control, # P < 0.05 versus t-BHP, and ## P < 0.01 versus t-BHP. (b) Real-time PCR analyses of the mRNA expression of HO-1, NQO1, and GCLM in ARPE-19 cells in the absence of t-BHP. Significance: # P < 0.05 versus control siRNA. (c) Western blot analyses of protein abundance of HO-1, NQO1, and GCLM in t-BHP-treated ARPE-19 cells with quantification. Significance: ∗ P < 0.05 versus control, # P < 0.05 versus t-BHP, and ## P < 0.01 versus t-BHP. (d) Western blot analyses of protein abundance of HO-1, NQO1, and GCLM in ARPE-19 cells in the absence of t-BHP with quantification. Significance: # P < 0.05 versus control; ## P < 0.01 versus control.