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. 2016 Sep 14;6:33235. doi: 10.1038/srep33235

Figure 1. Scheme of the recombination event.

Figure 1

The Xs (double crossover) represent the gene exchange sites between the ΦV10 genome and the PCR amplicon containing the kanR-nluc cassette amplified by primers pv10recdnluc-F and pv10recdnluc-R. The absence of recET and the presence of kanR-nluc cassette flanked by the homologous sequences 5′-GTGGCGCCGCGGTGGTGGTTACATAGATGTTTCGTTT-3′ and 5′-TCGCCCGCTGGGATCTGGCGGATCAGCTTGATGGAC-3′ (shown in shaded bars) in the ΦV10 genome was confirmed by sequencing using primers pv1035-R and pv1039-F. The map was drawn based on the sequence of the Enterobacteria phage PhiV10 (GenBank accession NO. DQ126339.2).