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. 2016 Sep 14;6:33235. doi: 10.1038/srep33235

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Wild type E. coli O157:H7 C7927 was inoculated at 5.40 × 10⁸ (), 5.40 × 10⁷ (), 5.40 × 10⁶ (), 5.40 × 10⁵ (), 5.40 × 10⁴ (), 5.40 × 10³ (), 5.40 × 10² (), 5.40 × 10¹ (), 5.40 × 10⁰ () CFU per assay (in a total of 40 ml), and the threshold (—) was set at double the background value. The phage added per assay was 1.76 × 10² pfu ml⁻¹. At 1 hour intervals, individual light readings were recorded for triplicate samples. The cutoff of the bioluminescence reading was set up at 2.1 × 10⁷ photons per second which is the upper detection limit of the Sirius luminometer used in this study.

Inline graphic — Wild type E. coli O157:H7 C7927 was inoculated at 5.40 × 10⁸ (), 5.40 × 10⁷ (), 5.40 × 10⁶ (), 5.40 × 10⁵ (), 5.40 × 10⁴ (), 5.40 × 10³ (), 5.40 × 10² (), 5.40 × 10¹ (), 5.40 × 10⁰ () CFU per assay (in a total of 40 ml), and the threshold (—) was set at double the background value. The phage added per assay was 1.76 × 10² pfu ml⁻¹. At 1 hour intervals, individual light readings were recorded for triplicate samples. The cutoff of the bioluminescence reading was set up at 2.1 × 10⁷ photons per second which is the upper detection limit of the Sirius luminometer used in this study.