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. 2016 Sep 14;6:33235. doi: 10.1038/srep33235

Table 2. The oligonucleotide primers used for this study.

Primer Nucleotide sequence (5′-3′)
nluc-F ATGGTCTTCACACTCGAAGATTTCGTTGG
nluc-R CCGACTCTAGAGTCGCGGCCTTACGCCAGAATGCG
nluc-F′a ATTAACTTTATAAGGAGGAAAAACATATGGTCTTCACACTCGAAGATTTCG
pv10recdnluc-Fb TCGCCCGCTGGGATCTGGCGGATCAGCTTGATGGACCCATCATCGATGAATTGTGTC
pv10recdnluc-Rc GTGGCGCCGCGGTGGTGGTTACATAGATGTTTCGTTTCCGACTCTAGAGTCGCGGCCTTACGCCAGAATGCG
pv1039-F ATGCAGTGGAAAATCATC
pv1035-R GTCGGCTTAACTTTCTCAC

aThe sequence of the translation initiation region was shown in bold letters.

bThe sequence underlined was homologous to the upstream of the kanamycin resistant determinant.

cThe sequence underlined was homologous to the downstream of the nluc gene.