Table 2. The oligonucleotide primers used for this study.
Primer | Nucleotide sequence (5′-3′) |
---|---|
nluc-F | ATGGTCTTCACACTCGAAGATTTCGTTGG |
nluc-R | CCGACTCTAGAGTCGCGGCCTTACGCCAGAATGCG |
nluc-F′a | ATTAACTTTATAAGGAGGAAAAACATATGGTCTTCACACTCGAAGATTTCG |
pv10recdnluc-Fb | TCGCCCGCTGGGATCTGGCGGATCAGCTTGATGGACCCATCATCGATGAATTGTGTC |
pv10recdnluc-Rc | GTGGCGCCGCGGTGGTGGTTACATAGATGTTTCGTTTCCGACTCTAGAGTCGCGGCCTTACGCCAGAATGCG |
pv1039-F | ATGCAGTGGAAAATCATC |
pv1035-R | GTCGGCTTAACTTTCTCAC |
aThe sequence of the translation initiation region was shown in bold letters.
bThe sequence underlined was homologous to the upstream of the kanamycin resistant determinant.
cThe sequence underlined was homologous to the downstream of the nluc gene.