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. 2016 Sep 14;6:33235. doi: 10.1038/srep33235

Table 3. Bacterial strains, phages and plasmids used and constructed in this study.

Bacteria/Phage/Plasmid Description Source or reference
Bacteria
 E. coli O157:H7 C7927 Human isolate, host cell for propagation of ΦV10 and its mutant 32
 E. coli O157:H7 C7927 (ΦV10) A ΦV10 lysogenic strain Bruce Applegate
 E. coli O157:H7 C7927 (ΦV10nluc) A ΦV10nluc lysogenic strain This study
 OneShot TOP10 General E. coli cloning host Invitrogen
Phage
 ΦV10 Non-virulent phage, E. coli O157:H7 specific 15
 ΦV10nluc ΔrecET::kanRnluc mutant strain of ΦV10 This study
Plasmids
 pGEM-T Easy General cloning vector Promega
 pNL1.1 NanoLuc luciferase reporter vector Promega
 pNluc The nluc gene with a translation initiation region cloned into pGEM-T Easy vector This study
 pFSP138 The kanamycin resistance determinant of Tn5 cloned into pCR 2.1 TOPO vector Bruce Applegate
 pNluc-kan pFSP138 with insertion of kanR determinant upstream of nluc This study
 pKD46 A lambda Red recombinase plasmid 30