Figure 6. Change of hepatic Kv 3.2 expression in obese mice and palmitate treated liver cells.

(A,B) Whole lysate was extracted from liver tissue of 7 week-old male ob/ob with/without high-fat diet for 3 weeks and age-matched lean mice; western blotting was performed. (C) Sk-Hep I cells treated with palmitate (500 uM) for 24 h. The expression levels of protein and transcription factors related to gluconeogenesis and the ER stress response were analyzed in whole cell lysate by western blotting. (D) Kv 3.2 expression was analyzed in whole cell lysate by western blotting. *Presents significant differences between groups at p < 0.05. All data were represented as means ± SD, n = 3. Middle-length blots and two exposures are presented in Supplementary Figure 5.