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. 2016 Sep 14;6:33396. doi: 10.1038/srep33396

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Copyright © 2016, The Author(s)

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(a) Effect of cysteine replacements on Prx activity of Asp f3 measured in the presence of H₂O₂ or t-bOOH with Ampliflu Red. (b) Reaction schematics of the Ampliflu Red assay for Prx activity. (c) Reaction kinetics of Asp f3 and mutants measured in the presence of increasing concentrations of H₂O₂ or t-bOOH with Ampliflu Red. (d) NADPH-dependent Prx activity of Asp f3 and Cys replacement mutants determined in the presence t-bOOH, GSH, GSHR, monitored as a decline in NADPH light absorbance at 340 nm. (e) Reaction scheme of the NADPH-based Prx activity assay.