Figure 4. Asp f3 protects from peroxide stress.

(A) Preinoculated conidia of A. fumigatus D141 (WT), the asp f3 deletion mutant (∆asp f3) and the complemented strain (asp f3^C) were grown for 18 h at 37 °C during agar diffusion of 50 μl of 300 mM (left) or 75 mM (right) H₂O₂, of 30 mM t-bOOH, and 1 mM (left) or 0.25 mM (right) of menadione. (B–D) Columns give the average size of inhibition zones (mm) with distinct concentrations of each stress factor determined for all strains in four biological replicates; error bars show standard deviation. (E) Growth of fungal strains as in (A–D) in the presence of different concentrations of H₂O₂. Values are expressed as average percentages of calcofluor white fluorescence relative to untreated controls in three biological replicates. (F) Fluorescence micrographs of ∆asp f3 after 24 h of growth in the absence (0 μM) or presence of 100, 250, and 500 μM of H₂O₂ following chitin specific staining with calcofluor white. Bars are 100 μm.