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. 2016 Sep 14;6:33412. doi: 10.1038/srep33412

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Reaction catalyzed by PglC to produce Und-PP-diNAcBac and release UMP as a by-product. (B) Detection of the UMP by-product by the UMP-Glo assay. UMP is converted to a luminescence signal by the UMP-Glo reagent.