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. 2016 Jul 18;37(9):1208–1217. doi: 10.1038/aps.2016.52

Figure 6.

Figure 6

The effects of Psm2 on PI3K signaling. (A) Gel-filtered platelet suspensions were preincubated with Psm2 (0.3 mg/mL), TGX-221 (10 μmol/L) or vehicle, followed by stimulation with ADP (10 or 20 μmol/L), U46619 (3 or 6 μmol/L), collagen (2 or 4 μg/mL) or thrombin (1 or 2 U/mL). The phosphorylation of Akt was detected by Western blotting. (B) The platelets were treated with Psm2 (0.3 mg/mL) or vehicle and then were stimulated by thrombin (1 U/mL). The phosphorylation of Akt was measured 0, 20, 60, or 180 s after thrombin stimulation. n=3. Mean±SEM. *P<0.05, **P<0.01 vs the agonist-induced group. NS indicates no significance.