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CCL17 and CCL19 increase the number of mature DCs in secondary lymphoid tissues. Splenocytes and DLN cells were collected from mice treated with pCCL17/VAX, pCCL19/VAX, pCCL17/VAX plus pCCL19/VAX, or pVAX1, digested with collagenase IV 3 days after immunization, stained with a DC marker (CD11c) and a class II marker (I-Ab α chain), and analyzed by flow cytometry. This result is representative of three experiments.
