Fig. 2.

Relationship between reads and capture target bases. a Relationship between reads and numbers of covered capture target bases. The numbers of captured target nucleotide bases are plotted against total number of sequence reads obtained in 375 trees from exome capture sequencing. The linear regression coefficient (r ²) is 0.23 (P < 0.001). b Distribution of on-target coverage ≥ 10x depth across the 375 trees. The numbers of capture target bases with a coverage depth of ten or greater sequence reads per target are plotted against the total number of sequence reads. The relationship seemed approximately linear within a limited range of the total number of reads at 37–55 million. c Distribution of mean coverage depth across the 375 trees. The mean coverage depth is plotted against the total number of sequence reads. The linear regression coefficients (r ²) was significant (P < 0.001) and equalled 0.72