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. 2016 Sep 13;15(1):155. doi: 10.1186/s12934-016-0556-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Ergosterol biosynthesis. a RP-HPLC ergosterol analysis from the parental host strain, ΔCYP61(+, −) and ΔCYP61(−, −). Chromatograms (at 280 nm) correspond to ergosterol extracted from strains as described in the “Methods” section. Ergosterol extracts (black) from the parental host strain, ΔCYP61(+, −) strain and ΔCYP61(−, −) strain were analyzed with co-injection of ergosterol standard (pink). b Ergosterol production in the parental host strain, ΔCYP61(+, −) strain and ΔCYP61(−, −) strain. Circle (black), triangle (blue) and square (red) symbols represent values of the parental host strain, ΔCYP61(+, −) strain and ΔCYP61(−, −) strain, respectively. The values are means and the error bars show the standard deviation (n = 3)