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. 2016 Sep 14;7(1):133. doi: 10.1186/s13287-016-0384-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Western blotting validations of mass spectrometry results. COMP, biglycan and SOD1 abundance were confirmed by western blotting. Representative western blots for tenogenic constructs (a COMP and c biglycan) and chondrogenic constructs (e SOD1). Abundance of each protein is expressed semi-quantitatively (b, d, f) relative to total protein content. Statistical differences were assessed with age in the respective construct and antibody analysis using Mann–Whitney tests. Significant differences represented with p ≤ 0.05