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. 2016 Sep 13;18(1):203. doi: 10.1186/s13075-016-1099-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Immunofluorescent staining for triggering receptor expressed on myeloid cells (TREM)-2 and macrophage (CD14) as well as colocalization of TREM-2 with CD14⁺ cells in articular cartilage and synovial membrane. Immunofluorescent staining for TREM-2 (a, e, i, m, q, u), CD14 (b, f, j, n, r, v), 4′,6-diamidino-2-phenylindole (DAPI) (c, g, k, o, s, w), and merged images for examining colocalization of TREM-2 and CD14 (d, h, l, p, t, x). Arrows indicate colocalization of TREM-2 with CD14. These are the representative images of five swine each in the vitamin D-deficient (VDDef) and vitamin D-sufficient (VDSuff) groups and three swine in the vitamin D-supplemented (VDSupp) group