Table 1.
LOD, ng/mL | LOQ, ng/mL | extraction efficiency (“free”/”total”), % | matrix effect (“free”/”total”), % | |||||
---|---|---|---|---|---|---|---|---|
analyte | “free”/“total”a | “free”/“total” | lowb | mid | high | low | mid | high |
OHTHC | 0.008/0.017 | 0.028/0.048 | 93/69 | 81/67 | 82/75 | 1.0/13 | −5/1 | −9/−32 |
COOH-THC | 0.005/0.015 | 0.018/0.045 | 83/58 | 81/60 | 83/59 | 5/9 | −4/−6 | −2/−41 |
CBD | 0.004/0.009 | 0.012/0.030 | 88/22 | 70/26 | 71/28 | 8/23 | −9/−4 | −5/−9 |
CBN | 0.002/0.007 | 0.008/0.023 | 76/20 | 64/18 | 71/21 | 2/−6 | −8/−16 | −4/−12 |
THC | 0.002/0.005 | 0.008/0.015 | 74/18 | 59/19 | 54/22 | 6/32 | −7/−5 | −7/−12 |
“Free” and “total” refer to unconjugated and the sum of conjugated and unconjugated forms, respectively.
Low, mid and high levels refer to 0.025, 25, and 500 ng/mL, respectively.