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. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Virus Res. 2015 Jan 30;200:19–23. doi: 10.1016/j.virusres.2015.01.018

Figure 2. Effect of resveratrol and quercetin on viral replication.

Figure 2

A549 cells were either mock-infected (Ctrl) or infected with hMPV at MOI of 1 for 24h in the presence or absence of 50 µM resveratrol or 10 µM quercetin, and harvested to prepare (A) total RNA to quantify RSV N gene expression by qRT-PCR; and (B) total cell lysates for western blot analysis for hMPV viral protein expression. For loading controls, membrane was stripped and reprobed with anti β-actin antibody; (C) A549 cells infected with hMPV at MOI of 1 for 24h and treated or untreated with various doses of resveratrol (10 – 50 µM in DMSO) or quercetin (1 – 10 µM in DMSO) or vehicle alone were harvested to measure viral titer by plaque assay. Results are expressed as mean ± standard error and are representative of three independent experiments. *P < 0.05 relative to hMPV-infected cells.