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. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Virus Res. 2015 Jan 30;200:19–23. doi: 10.1016/j.virusres.2015.01.018

Figure 3. Effect of resveratrol and quercetin on transcription factor activation.

Figure 3

A549 cells were infected with hMPV at MOI of 1 for 24h in the presence or absence of 50 µM resveratrol or 10 µM quercetin. (A) Nuclear proteins were analyzed for NF-κB/p65 and IRF-3 levels by Western blot. For loading controls, membranes were stripped and reprobed with anti lamin B antibody. (B) ChIP-QgPCR analysis of p65 occupancy of the IL-8 gene promoter NF-κB site (left panel) and IRF-3 occupancy of the RANTES gene promoter ISRE site (right panel). Data shown is representative of three independent experiments. *P < 0.05 relative to 24h hMPV infected cells.