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. 2005 Mar 15;7(8):1485–1487. doi: 10.1021/ol050081+

Figure 1.

Figure 1

Radiolabeled annealed primer/template (250 nM). Position 1 in each template (lanes 2−5) provided the corresponding Watson−Crick complementary deoxynucleoside to test elongation of the DNA primer by one residue. Each reaction included 20 mM Tris-HCl, 10 mM KCl, 10 mM (NH4)2SO4, 20 mM MgSO4, 0.1% Triton X-100, 0.2 μg/μL BSA, 100 μM DTT, 0.2 units (37 nM) of Therminator Polymerase, 1.25 mM Mn2+, and 0.5 μM tNTP and were incubated at 55 °C for 15 min. Lane 1:  Radiolabeled primer. Lane 2:  tGTP (no Mn2+). Lane 3:  tDTP. Lane 4:  tTTP. Lane 5:  tCTP. Lane 6:  tGTP, tDTP, tTTP, and tCTP with an incubation time of 120 min.