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. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Bone. 2013 Feb 4;54(1):157–168. doi: 10.1016/j.bone.2013.01.041

Fig. 6.

Fig. 6

Role of AMBN in BMMC adhesion. BMMCs were seeded onto culture plates that were coated with AMBN or AMEL at two different concentrations, and blocked with BSA. (A) Adhesion of BMMCs on BSA, AMBN or AMEL coated plates. The number of attached cells was counted after 4 h of culture. AMBN5/AMEL5 contained 5 μg/ml and AMBN10/AMEL10 contained 10 μg/ml of the recombinant AMBN or AMEL in the coating solution used to coat the plates. (B) Western blot analysis of the effect of AMBN on adhesion-dependent phosphorylation of key extracellular matrix signaling molecules, ERK and AKT, in BMMCs. The Western blot compares BSA and AMBN treatments after 15 (AMBN15), 30 (AMBN30) and 45 (AMBN45)min of incubation. ***=pb<.0001.