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Effect of AMBN on osteoclast activation-related protein expression. BMMCs were seeded onto BSA or AMBN coated culture dishes and incubated for 1 h (Rho A 1 h), 4 h (Rho A 4 h) or 12 h (NFATc1 and c-FOS) in the presence of M-CSF and RANKL. AMBN function was selectively inhibited with anti-α2β1 integrin antibody or ERK inhibitor. Expression of osteoclast activation-related proteins was detected by Western blot. β-Actin served as loading control.
