Figure 2.

Pneumonia can induce secreted and transmembrane (Sectm) 1 transcription in epithelial cells by mechanisms other than RelA. (A and B) C57BL/6 mice were infected with Streptococcus pneumoniae serotype 19F. mRNAs were measured in left-lung homogenates using quantitative RT-PCR (qRT-PCR) at indicated time points. Values represent fold induction relative to 0 hours and were expressed as means (±SEM). Significance was determined by one-way ANOVA with Sidak’s post hoc test (n = 4–8 per group). *P < 0.05 versus 0 hours. (C and D) NK2 homeobox 1–Cre Rela-floxed mice in which RelA was mutated throughout the lung epithelium, or wild-type (WT) control littermates with no Cre expression, were infected with pneumococcus and killed 9 or 24 hours after infection, after which left-lung epithelial cells, identified as CD45⁻EpCAM⁺, were isolated by FACS. Values represent fold induction relative to epithelial cells isolated from 0-hour Cre-negative mice and were expressed as mean (±SEM). Significance was determined by two-way ANOVA with Sidak’s post hoc test (n = 3–4 per group). *P < 0.05 versus 0 hour.