Figure 5.

Assessment of HDM-stimulated Il-5 and Il-17A cytokine in single-cell suspensions prepared from lung tissue or spleens isolated from WT or Glrx1^−/− mice exposed to PBS or HDM. Media were collected from single–lung cell (A and B) or spleen suspensions (C and D) from unstimulated (top) and cells restimulated (bottom) for 96 hours with 15 μg/ml of HDM. IL-17A and IL-5 production was quantified by ELISA. Data are expressed as means (± SEM; six mice per group) and are representative of three independent experiments. *P < 0.05 (ANOVA) compared with PBS controls; ^†P < 0.05 (ANOVA) compared with WT mice challenged with HDM.