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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Immunol. 2016 May 18;197(1):68–77. doi: 10.4049/jimmunol.1501297

Figure 4. TNF-α, IL-17a, and IL-8 production following direct TLR-2 co-stimulation of neonatal and adult naïve CD4+T cells.

Figure 4

Purified neonatal (A) and adult (B) naïve CD4+CD45RA+ T cells were stimulated as indicated in triplicate. Culture supernatants were harvested at 18 h (TNF-α, IL-8 and 72 h (IL-17a) and cytokine production measured by ELISA. Cytokine data was log transformed with base 2 for analysis due to the skewed distribution of data. Shown are log 2 transformed mean group responses ± SD from 6 neonatal and 6 adult donors. Statistical analysis was performed exactly as described in Figure 2. Adjusted p values < 0.05 were considered significant and are indicated in figure.