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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Immunol. 2016 May 18;197(1):68–77. doi: 10.4049/jimmunol.1501297

Table II.

Expression of activation markers by naïve CD4+ T cells from neonatal and adult donors following TLR-2 co-stimulation1

Rest αCD3 αCD3 + P3C (10 μg/ml) αCD3 + αCD28 αCD3+ αCD28 + P3C (10 μg/ml)
Neonate Adult Neonate Adult Neonate Adult Neonate Adult Neonate Adult
CD25 2.6 ± 1 2.4 ± 1 29 ± 7 23 ± 8 53 ± 8* 39* ± 9 31* ± 7 28 ± 9 55 ± 7* 40 ± 10*
CD69 0.2 ± 0.1 0.3 ± 0.1 40 ± 8* 59 ± 5* 57 ± 8* 62 ± 5* 40 ± 9* 61 ± 6* 56 ± 9* 64 ± 4*
CD154 1.1 ± 1 0.2 ± 0.1 24 ± 8 29 ± 4 49 ± 14* 48 ± 9* 24 ± 8 28 ± 5 50 ± 14* 47 ± 10*
1

Expression of individual activation markers (CD25, CD69, and CD541) on highly purified naïve CD4+ T cells from neonatal and adult donors stimulated for 18 h under the indicated conditions (shown are mean percentage ± SEM from 8 experiments using 8 adult and 8 cord donors). There were no significant differences in expression of any activation markers under the tested conditions between neonatal and adult donors.

*

Indicates p-value < 0.01 for within age-group comparison of stimulated to resting condition using two-way ANOVA followed by Tukey-Kramer adjustment for multiple comparisons.