Table 1. Bacterial strains and plasmids.
| Description | Source or reference | |
|---|---|---|
| Strains | ||
| B. bacilliformis | CIP 57.20 –NCTC12136 | Institute Pasteur |
| E. coli TOP10 | Host strain for cloning | Invitrogen |
| E. coli BL21Star (DE3) | Host strain for gene expression | Invitrogen |
| Plasmids | ||
| pCR4-TOPO | TA-cloning vector | Invitrogen |
| pET100D/TOPO | Expression vector | Invitrogen |
| pGroEL | pET100D/TOPO containing GroEL | This study |
| pPap31 | pET100D/TOPO containing Pap31 | This study |
| pSCS-α | pET100D/TOPO containing SCS-α | This study |
| pSCS-β | pET100D/TOPO containing SCS-β | This study |
SCS-α: succinyl-CoA synthetase subunit α
SCS-β: succinyl-CoA synthetase subunit β.