Table 1. Information of all primers.

Target			Sequence 5'-3'	Amplicon size(bp)
1	FluA	F	GTACGACTCACTATAGGGATGGACAAAKCGTCTACGCTGC	140
		R	AGGTGACACTATAGAATAGACAAGACCAATTCTGTCACCT
2	ADV	F	GTACGACTCACTATAGGGAGCTGGACATGACYTTYGAGGT	144
		R	AGGTGACACTATAGAATAGATGACGCCGCGGTGYGGCT
3	PIV1	F	GTACGACTCACTATAGGGATGATGAATACGCATATATTG	152
		R	AGGTGACACTATAGAATACAATATCTCATTATTACCYGG
4	HBoV	F	GTACGACTCACTATAGGGATTGGAGAAATCACTGCTACTG	156
		R	AGGTGACACTATAGAATAGAAATGAGTTTGGAATTTTA
5	HRV	F	GTACGACTCACTATAGGGAGTAGCACACGSGGCTCTT	162
		R	AGGTGACACTATAGAATACCTGGCAGATGAGGCWG
6	229E	F	GTACGACTCACTATAGGGATTCTTTTAGAACATCACAATTTTGTTCATC	182
		R	AGGTGACACTATAGAATACTGAGCATGATTTCTTTACTTGGAAAG
7	SARS	F	GTACGACTCACTATAGGGACTTAGCTACTTCRTTGCTTCY	199
		R	AGGTGACACTATAGAATAGAATGATCACAGCACCAATGA
8	N2	F	GTACGACTCACTATAGGGAGCACAGTAGTAATGACTGATG	202
		R	AGGTGACACTATAGAATACAGACACAYCTGACAYCAGG
9	FluB	F	GTACGACTCACTATAGGGACTGAAATGGTTCGAGCATTATAG	207
		R	AGGTGACACTATAGAATAAAGCTGGCAGAARAGYTGCA
10	PIV3	F	GTACGACTCACTATAGGGAAGAAGGAAGATTACTTYTACT	213
		R	AGGTGACACTATAGAATACCCATGGACATTCRTTGTTTC
11	H5N1	F	GTACGACTCACTATAGGGAGGCWATAGATGGAGTCACC	217
		R	AGGTGACACTATAGAATACATGAGAACCAGAAGTTCAGCATT
12	PIV2	F	GTACGACTCACTATAGGGAAACCATTTACCTAAGTGATGG	225
		R	AGGTGACACTATAGAATAGATATGAATCTTTCAATAAAGG
13	HMPV	F	GTACGACTCACTATAGGGATGCCTCTTAAGAGAYGAYCARG	235
		R	AGGTGACACTATAGAATATGTGCTAACTTTGCAYGGGTARTT
14	swH1N1	F	GTACGACTCACTATAGGGATTGAGCTCAGTRTCATCWTTTGAA	239
		R	AGGTGACACTATAGAATATCCCTTTATCATTAATGTAGGATTTGC
15	RSV	F	GTACGACTCACTATAGGGAATTACCAAGTGARGTAARTCTCTGCA	246
		R	AGGTGACACTATAGAATAATACATAATCACACCCGTTAGARAA
16	SeH1N1	F	GTACGACTCACTATAGGGATATGCTTTTGCARTGAYTAGAMG	253
		R	AGGTGACACTATAGAATAAAGGGATATTCCTTAYTCCTGTAAMC
17	H1	F	GTACGACTCACTATAGGGAGCYGAYCARAAGAGCACACAYAAT	260
		R	AGGTGACACTATAGAATACCAAAGTRCTTTCATYTTCCAYT
18	H3	F	GTACGACTCACTATAGGGAGCTGGTTCAGAGTTCCTCAACA	277
		R	AGGTGACACTATAGAATAAAACTCCAGTKTGCCKGATGA
19	OC43	F	GTACGACTCACTATAGGGAATCCCAWTGACAATCRAASGG	290
		R	AGGTGACACTATAGAATAGAATGTTGCTAAGTAYACTCAYTTA
20	Internal Control	F	GTACGACTCACTATAGGGAGCCAGATATACGCGTTGACA	322
		R	AGGTGACACTATAGAATAGGGCGTACTTGGCATATGAT
21	N1	F	GTACGACTCACTATAGGGAGGRGCCTTGYTYAATGRCA	330
		R	AGGTGACACTATAGAATAACACATGCACATTCAGAYTCTYG

The underlined sequences were the universal Tag sequences.