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. 2016 Sep 14;11(9):e0162858. doi: 10.1371/journal.pone.0162858

Fig 2. PDGFRα+ cells recombine at low efficiency in endocrine tissue.

Fig 2

Cryosections through the adrenal and pituitary gland of P57+7 Pdgfrα-CreERT2::Rosa26-YFP were immunostained to detect PDGFRα (red), YFP (green) and Hoescht 33342 (blue). Confocal image (single z plane) of the adrenal gland at low (a) and high (b) magnification. Confocal image (single z plane) of the pituitary gland at low (c) and high (d) magnification. (e) The number of PDGFRα+ YFP+, PDGFRα+ YFP-negative and PDGFRα-negative YFP+ cells quantified from confocal images (single z plane) of the adrenal and pituitary glands, expressed as the number of cells per mm2. (f) The proportion of PDGFRα+ cells that become YFP-labelled (PDGFRα+ YFP+ cells / total PDGFRα+ cells, x 100) quantified from confocal images (single z plane) of adrenal and pituitary gland. Error bars represent mean ± std dev from n = 3 mice. PDGFRα+ YFP+ cells are denoted by arrowheads. PDGFRα-negative YFP+ cells are denoted by arrows. PDGFRα+ YFP-negative cells are denoted by small arrows. Scale bars represent 50μm.