Fig 3. Susceptibility assays to reveal functional indispensability of calcineurin against PA in C. albicans.
(a) Spot assay with and without PA (175 μg ml-1) as controls. (b) Spot assay with PA at alkaline pH10. (c) Spot assay with PA in serum (50% w/v). (d) Spot assay with PA under ER stress by DTT (20mM). (e) Spot assays with PA under various ionic stress conditions with LiCl (0.4M), CaCl2 (0.3M), NaCl (1M). (f) Spot assay with PA in presence of membrane perturbing agent SDS (0.02% w/v). (g) Spot assay with PA at elevated temperatures of 37°C and 42°C. (h) Spot assay depicting loss of growth in Δcnb1 mutant in the presence of PA (225μg ml -1) while the Δcrz1 mutant and CNB1-1/CNB1 (calcineurin strain with constitutively expressed hyperactive allele of CNB1) were efficiently growing in presence of PA.