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. 2016 Sep 14;11(9):e0162465. doi: 10.1371/journal.pone.0162465

Fig 7. Effect of PA on virulence traits of C. albicans.

Fig 7

(a) Effect of PA on Dimorphic Switching of C. albicans. Left panel shows hyphal morphogenesis in the liquid hyphal inducing media (YEPD, Spider media and YEPD with 10% Serum) in the absence (control) and presence of PA (225μg ml-1) in C. albicans at 4 hours (Magnification 40X). Right panel shows hyphal morphogenesis in the solid hyphal inducing media (SLAD medium, Spider medium and 10% Serum) in the absence (control) and presence of PA (225μg ml-1) in C. albicans at 4 hours (Magnification 4X). (b) SEM showing the biofilm formation in absence (control) and presence of PA (175μg ml-1). (c) Effect of PA (175μg ml-1) on biofilm formation of C. albicans depicted as bar graph and quantified by using MTT assay as described in material and methods. Mean of OD450 ± SD of three independent sets of experiments is depicted on Y-axis and * depicts P value <0.05. (d) Effect of PA (175μg ml-1) on cell adherence of C. albicans quantified by using MTT assay as described in material and methods. Mean of O.D450 ± SD of three independent sets of experiments is depicted on Y-axis and * depicts P value <0.05. (e) Effect of PA (175μg ml-1) on adherence of C. albicans to the buccal mucosal epithelial cell. Left panel shows Candida cells adhered to the epithelial cells marked by an arrow and inhibitory effect of PA showing no adherence in right panel.