Fig 4. ARQ 087 arrests cells in the G1 cell cycle phase and induces apoptosis.

(A) NCI-H716 and SNU-16 cells were treated with 1 μM of ARQ 087 or vehicle for 72 hours. Cell cycle profiles were measured by flow cytometric analyses. (B) SNU-16 cells were treated with ARQ 087 (1 mM) for 0, 24, 48 and 72 hrs. Western blot analysis was performed for XIAP, cleaved-PARP (c-PARP), activated-caspase 3 (a-Caspase 3), and phospho-p53 were assessed by Western blot analyses. β-Actin was used as the loading control.