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. 2016 Sep 14;11(9):e0162946. doi: 10.1371/journal.pone.0162946

Fig 1. Baculovirus construct for production of chimeric virus-like particles.

Fig 1

(A) construction of a F/HA fusion gene containing ectodomain of the NDV KR-005/00 strain F gene and TM/CT domain of the H5N1 avian influenza virus ES03 strain HA gene. The schematic diagram shows the location of the ectodomain, transmembrane (TM) domain, and cytoplasmic tail (CT) domain of the F and HA genes and their junction. The TM/CT domain of the HA gene was fused to the carboxyl terminal of the F gene to form the full-length F/HA fusion gene; (B) HA, M1, and chimeric F/HA genes with its own polyhedrin promoter (Pph) and transcription termination sequences are indicated.