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. 2016 Sep 14;11(9):e0162946. doi: 10.1371/journal.pone.0162946

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© 2016 Noh et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Analysis of concentrated chimeric VLP: (A) Western blot identification of the HA protein using anti-H5 monoclonal antibody. M, standard molecular size marker (EBM-1032, Elpisbio), HA, chimeric VLPs; (B) Western blot identification of the F/HA chimeric protein using anti-NDV F monoclonal antibody; (C) Western blot identification of the M1 protein using anti-H5N1 anti-serum; (D) TEM identification of chimeric VLP with scale bar; (E) immunogold-labelled chimeric VLP: VLP were probed with anti-H5 monoclonal antibody, counterstained with 10nm gold spheres coupled to anti-mouse IgG and anti-NDV anti-serum, counterstained with 25nm gold spheres coupled to anti-chicken IgG.