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. 2016 Sep 14;11(9):e0162946. doi: 10.1371/journal.pone.0162946

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© 2016 Noh et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) HI titers after three weeks post vaccination and two weeks after HPAIV challenge: serum samples were analyzed with the standard HI test for the presence of HI antibodies against ES03 H5 AIV. Error bars represent standard deviations. ***, P < 0.001, **, P < 0.01, (B) Survival curve after lethal HPAIV challenge. Three weeks after immunization, SPF chickens were intranasally infected with a lethal dose of ES03 HPAI virus. The chickens were observed daily for a period of 14 days for survival. (C) Differentiation of vaccinated chickens from vaccinated and infected chickens. Serum samples were taken before challenge (3 weeks post-vaccination) and 14 days post challenge. NP antibody levels were tested with a commercially available NP-cELISA kit. Each dot represents the NP-specific antibody value of each chicken.