Table 1. Primer sets used for RT-PCR amplification of genes of HPAIV ES03 and NDV KR-005/00.
| Target gene | Primer (5’-3’) | Expected size(bp) |
|---|---|---|
| HA | BamHI-HA-1:GGA TCC ATG GAG AAA ATA GTG C | 1695 |
| HindIII-HA-2:AAG CTT AGT AGA AAC AAG G | ||
| M1 | EcoRI-M1-1:GAA TTC ATG AGT CTT CTA ACC GAG G | 759 |
| HindIII-M1-2:AAG CTT TCA CTT GAA TCG CTG C | ||
| F(TM/CT removed) | EcoRI-F-1:GAA TTC ATG GGC TCC AAA CTT TC | 1500 |
| XhoI-F-2:CTC GAG AGC AGA TGT GCT GGT TAG | ||
| HA TM/CT | XhoI-HA-TM/CT-1:CTC GAG CAG ATC CTG TCC ATC TAC | 117 |
| HindIII-HA-TM/CT-2:AAG CTT TTA GAT GCA GAT ACG GCA C |