Table 1.
Bacteriocin-related primer sequences used in this study.
| Primer | Target Gene | Sequence (5′→3′) | Product Size (bp) | Included Genes | Homologous Genes |
|---|---|---|---|---|---|
| 1 | Avicin A (avcA)—FJ851402.1 | 236 | avicin A precurson (avcA) immunity protein (avcI) divergicin-like bact (avcB) | sakacin X (sakX) | |
| Forward | ACG CGA AAT GAA GAA TGT TG | ||||
| Reverse | TTT CAT TTC CGC CAG AAA AC | ||||
| 2 | Columbicin A (colA)—EF033111.1 | 299 | columbicin A (colA) hypothetical protein (orfB) | bovicin A (bovA) enterocin AS-48 |
|
| Forward | TTT TTC TTG GGT TAT TTA CAG GAA | ||||
| Reverse | ATG TGC AAT GGG CAA AAA CT | ||||
| 3 | Durancin Q (duqQ)—AB284369.1 | 384 | immunity protein (duqI) durancin Q (duqQ) inducing peptide (duqF) | durancin TW-49 (durM) | |
| Forward | GCA CTG ATT CCG GCA CTA AT | ||||
| Reverse | CGT AAC TCT AAT GGC GGG AAG | ||||
| 4 | Enterocin 96 (ent96)—FJ769024.1 | 291 | enterocin 96 | - | |
| Forward | GTG GAG AGG ACG AAA GGA GA | ||||
| Reverse | TTG ATT AGT GGA GAG GAC GGT TA | ||||
| 5 | Enterocin mr10A/mr10B (mr10AB) | 247 | enterocin mr10A (mr10A) enterocin mr10B (mr10B) | enterocin JSB (entJSB) enterocin NA (entNA) enterocin NB (entNB) enterocin L50A (entL50A) enterocin L50B (entL50B) enterocin 62-6A (ent626A) enterocin 62-6B (ent626B) enterocin RJ-11 |
|
| Forward | ATG GGA GCA ATC GCA AAA T | ||||
| Reverse | CAT CCT TGT CCG ATA AAC TGC | ||||
| 6 | Enterocin C (entC)—FU862242.1 | 506 | enterocin C1 (entC1) enterocin C2 (entC2) enterocin C immunity (entCI) | enterocin 1081A enterocin 1071B |
|
| Forward | AGG TCC AGC TGC TTA TTG GA | ||||
| Reverse | CCA TTA GAA TGA ATA CGC TAA AGA AA | ||||
| 7 | Enterocin SE-K4 (entSE-K4)—AB092692.1 | 608 | enterocin SE-K4 (entSE-K4) enterocin precursor (orf7) entSE-K4 homologue (orf8) entSE-K4 immunity (orf9) | bacteriocin II (D78257.1) enterocin TW-21 bacteriocin 31 |
|
| Forward | ATG TAG AAG CCG CCA CGT AT | ||||
| Reverse | AAT CCC AAT CAT CCC ACA AA | ||||
| 8 | Enterocin EJ97(ej97a)—AJ490170.1 | 104 | enterocin ej97 | - | |
| Forward | AAA GCG ATG ATT AAG AAG TTT CC | ||||
| Reverse | TCC CAA GGA TAA CGA CCG TA | ||||
| 9 | Enterocin Wα/Wβ (enwAB)—AB600897.1 | 423 | enterocin W alpha (enwA) enterocin W beta (enw B) | - | |
| Forward | GGG GTT GAA TTA TTG TAG AAA GGA | ||||
| Reverse | AAC TAG CCT CTA CCG CCA CA | ||||
| 10 | Enterocin Q (entqA)—DQ832184.1 | 231 | enterocin Q (entqA) | - | |
| Forward | ATC ACA AAG TGA GCC CCT GT | ||||
| Reverse | TGG TAT CGC AAA ATG GAT GA | ||||
| 11 | Enterocin P (entP)—AF005726.1 | 431 | enterocin P (entP) enterocin P immunity (entQ) | - | |
| Forward | TTC CCC GAA GAA TAC AAA TGA | ||||
| Reverse | AAT TTC TGG GGT GGC TAA TG | ||||
| 12 | Enterocin A (entA)—AF240561.1 | 362 | enterocin A (entA) immunity protein (entI) | - | |
| Forward | AAA ATA AAT GTA CGG TCG ATT GG | ||||
| Reverse | CCA GCA GTT CTT CCA ATT TCA | ||||
| 13 | Enterocin B (entB)—U87997,1 | 257 | enterocin B (entB) | enterocin CRL35 | |
| Forward | CAG AGT TCC CAA CTG TTT GCT | ||||
| Reverse | AGC CCA TGC TAG TGG TCCT T | ||||
| 14 | Enterocin Xα/Xβ (enxAB)—AB430879.1 | 321 | enterocin X alpha (enxA) enteorcin X beta (enxB) | - | |
| Forward | GGACAATTTATGGGTAAACAAGC | ||||
| Reverse | TACGTCCACCATTCCAACCT | ||||
| 15 | Bacteriocin T8 (bacA)—AB178871.1 | 469 | bacteriocin precursor (bacA) hypothetical immunity protein (bacB) | hiracin JM79 Bac43 |
|
| Forward | TTGTCTAGCTGGCATCGGTA | ||||
| Reverse | CCAATAGAAGCCCATCCTCT | ||||
| 16 | Mundticin KS (munA)—KC291253.1 | 285 | mundticin KS (munA) | mundticin L (munL) enterocin HF |
|
| Forward | AAA AGG GTG CAG TGT TGA TTG | ||||
| Reverse | TCC ACT GAA ATC CAT GAA TGA | ||||